sds-page analysis of urinary proteins in dogs with heartworm disease

SDS-PAGE analysis of urinary proteins in dogs with heartworm disease

The aim of the study was to describe the urinary electrophoretic pattern of dogs with heartworm disease.Urine samples from 15 heartworm-infected and 15 healthy dogs were taken. Urinary specific gravity, urinaryprotein concentration and the urine protein/creatinine (U P/C) ratio were determined. Urine proteins werefractionated using SDS-PAGE. Results showed statistically significant differences ...

SDS-PAGE and Western blot of urinary proteins in dogs with leishmaniasis.

Canine leishmaniasis is an endemic disease in the Mediterranean area caused by the protozoan Leishmania infantum, which usually produces renal failure. Sodium dodecyl sulphate polyacrylamide gel electrophoresis and Western blot using antibodies to IgG and IgA from dogs were carried out in the urine of 22 dogs with leishmaniasis diagnosed by ELISA and confirmed by PCR, and 20 healthy dogs. The r...

Heartworm Disease in Dogs

The life cycle begins when a female mosquito bites an infected dog and ingests the microfilariae during a blood meal. The microfilariae develop further for 10 30 days in the mosquito's gut and then enter its mouthparts. At this stage, they are infective larvae and can complete their maturation when they enter a dog. The infective larvae enter the dog's body when the mosquito bites the dog. They...

Testing for heartworm disease in dogs.

Heartworm disease is caused by a parasite called Dirofilaria immitis, better known as heartworm. The parasites are long, hair-like worms that live in the right ventricle of the heart and the pulmonary artery, which is the large blood vessel that carries blood from the heart to the lungs. Dogs become infected when they are bitten by an infected mosquito that is carrying immature heartworms calle...

Study on pattern of Neospora caninumtachyzoite proteins by SDS-PAGE and Western blotting in aborted cows

The intracellular parasite Neospora caninumis prevalent in several countries and is increasingly recognized as an important cause of abortion and stillbirth in cattle. For characterizeing the tachyzoite antigens of Neospora caninumin aborted cows, sera were obtained from 116 cows which were aborted in the third semester of the pregnancy period and had antibodies to Neospora caninumin ELISA. To ...

Identification of electrophoretic proteins pattern of Fusaium verticillioides isolates from maize by SDS-PAGE.